Doctor Octopus, Master Planner

Other Villains you control get +2/+2.
Your maximum hand size is eight.
At the beginning of your end step, if you have fewer than eight cards in hand, draw cards equal to the difference.

Spider-Rex, Daring Dino

Reach, trample
Ward {2} (Whenever this creature becomes the target of a spell or ability an opponent controls, counter it unless that player pays {2}.)

Plains

Hunter's Insight

Choose target creature you control. Whenever that creature deals combat damage to a player or planeswalker this turn, draw that many cards.

Arachnogenesis

Create X 1/2 green Spider creature tokens with reach, where X is the number of creatures attacking you. Prevent all combat damage that would be dealt this turn by non-Spider creatures.

Web-Warriors

When this creature enters, put a +1/+1 counter on each other creature you control.

Behold the Sinister Six!

Return up to six target creature cards with different names from your graveyard to the battlefield.

Radioactive Spider

Reach, deathtouch
Fateful Bite — {2}, Sacrifice this creature: Search your library for a Spider Hero card, reveal it, put it into your hand, then shuffle. Activate only as a sorcery.

Iron Spider, Stark Upgrade

Vigilance
{T}: Put a +1/+1 counter on each artifact creature and/or Vehicle you control.
{2}, Remove two +1/+1 counters from among artifacts you control: Draw a card.

Lorthos, the Tidemaker

Whenever Lorthos attacks, you may pay {8}. If you do, tap up to eight target permanents. Those permanents don't untap during their controllers' next untap steps.

Gwenom, Remorseless

Deathtouch, lifelink
Whenever Gwenom attacks, until end of turn, you may look at the top card of your library any time and you may play cards from the top of your library. If you cast a spell this way, pay life equal to its mana value rather than pay its mana cost.

Beast Within

Destroy target permanent. Its controller creates a 3/3 green Beast creature token.

Spiders-Man, Heroic Horde

Web-slinging {4}{G}{G} (You may cast this spell for {4}{G}{G} if you also return a tapped creature you control to its owner's hand.)
When Spiders-Man enters, if they were cast using web-slinging, you gain 3 life and create two 2/1 green Spider creature tokens with reach.

Island

Counterspell

Forest

Venom, Deadly Devourer

Vigilance, menace
{3}: Exile target creature card from a graveyard. When you do, put X +1/+1 counters on target Symbiote, where X is the exiled card's toughness.

Kraven, Proud Predator

Vigilance
Top of the Food Chain — Kraven's power is equal to the greatest mana value among permanents you control.

Spider-Man Noir

Menace
Whenever a creature you control attacks alone, put a +1/+1 counter on it. Then surveil X, where X is the number of counters on it. (Look at the top X cards of your library, then put any number of them into your graveyard and the rest on top of your library in any order.)

Feed the Swarm

Destroy target creature or enchantment an opponent controls. You lose life equal to that permanent's mana value.

Infernal Grasp

Destroy target creature. You lose 2 life.

Swamp

Spider-Suit

Equipped creature gets +2/+2 and is a Spider Hero in addition to its other types.
Equip {3} ({3}: Attach to target creature you control. Equip only as a sorcery.)

Spider-Ham, Peter Porker

When Spider-Ham enters, create a Food token. (It's an artifact with "{2}, {T}, Sacrifice this token: You gain 3 life.")
Animal May-Ham — Other Spiders, Boars, Bats, Bears, Birds, Cats, Dogs, Frogs, Jackals, Lizards, Mice, Otters, Rabbits, Raccoons, Rats, Squirrels, Turtles, and Wolves you control get +1/+1.

Kraven's Last Hunt

(As this Saga enters and after your draw step, add a lore counter. Sacrifice after III.)
I — Mill five cards. When you do, this Saga deals damage equal to the greatest power among creature cards in your graveyard to target creature.
II — Target creature you control gets +2/+2 until end of turn.
III — Return target creature card from your graveyard to your hand.

Ezekiel Sims, Spider-Totem

Reach
At the beginning of combat on your turn, target Spider you control gets +2/+2 until end of turn.

Path to Exile

Exile target creature. Its controller may search their library for a basic land card, put that card onto the battlefield tapped, then shuffle.

Mountain

Arasta of the Endless Web

Reach
Whenever an opponent casts an instant or sorcery spell, create a 1/2 green Spider creature token with reach.

Spider-Man, Miles Morales

Vigilance, trample (Attacking doesn't cause this creature to tap. He can deal excess combat damage to the player he's attacking.)
Whenever Spider-Man enters or attacks, put a +1/+1 counter on each other creature you control. Those creatures gain trample until end of turn.