Aunt May

Whenever another creature you control enters, you gain 1 life. If it's a Spider, put a +1/+1 counter on it.

Arachne, Psionic Weaver

Web-slinging {W} (You may cast this spell for {W} if you also return a tapped creature you control to its owner's hand.)
As Arachne enters, look at an opponent's hand, then choose a card type other than creature.
Spells of the chosen type cost {1} more to cast.

Spider-Girl, Legacy Hero

During your turn, Spider-Girl has flying.
When Spider-Girl leaves the battlefield, create a 1/1 green and white Human Citizen creature token.

Island

Spider-Mobile

Trample
Whenever this Vehicle attacks or blocks, it gets +1/+1 until end of turn for each Spider you control.
Crew 2

Selfless Police Captain

This creature enters with a +1/+1 counter on it.
When this creature leaves the battlefield, put its +1/+1 counters on target creature you control.

Spider-Man India

Web-slinging {1}{G}{W} (You may cast this spell for {1}{G}{W} if you also return a tapped creature you control to its owner's hand.)
Pavitr's Sevā — Whenever you cast a creature spell, put a +1/+1 counter on target creature you control. It gains flying until end of turn.

Forest

Plains

Urban Retreat

This land enters tapped.
{T}: Add {G}, {W}, or {U}.
{2}, Return a tapped creature you control to its owner's hand: Put this card from your hand onto the battlefield. Activate only as a sorcery.

Radioactive Spider

Reach, deathtouch
Fateful Bite — {2}, Sacrifice this creature: Search your library for a Spider Hero card, reveal it, put it into your hand, then shuffle. Activate only as a sorcery.

Amazing Spider-Man

Vigilance, reach
Each legendary spell you cast that's one or more colors has web-slinging {G}{W}{U}. (You may cast a spell for its web-slinging cost if you also return a tapped creature you control to its owner's hand.)

Peter Parker

When Peter Parker enters, create a 2/1 green Spider creature token with reach.
{1}{G}{W}{U}: Transform Peter Parker. Activate only as a sorcery.

Peter Parker // Amazing Spider-Man

Hide on the Ceiling

Exile X target artifacts and/or creatures. Return the exiled cards to the battlefield under their owners' control at the beginning of the next end step.

Spider-Man, Web-Slinger

Web-slinging {W} (You may cast this spell for {W} if you also return a tapped creature you control to its owner's hand.)

Wall Crawl

When this enchantment enters, create a 2/1 green Spider creature token with reach, then you gain 1 life for each Spider you control.
Spiders you control get +1/+1 and can't be blocked by creatures with defender.

Spider-Man, Brooklyn Visionary

Web-slinging {2}{G} (You may cast this spell for {2}{G} if you also return a tapped creature you control to its owner's hand.)
When Spider-Man enters, search your library for a basic land card, put it onto the battlefield tapped, then shuffle.

Bagel and Schmear

Share — {W}, {T}, Sacrifice this artifact: Put a +1/+1 counter on up to one target creature. Draw a card. Activate only as a sorcery.
Nosh — {2}, {T}, Sacrifice this artifact: You gain 3 life and draw a card.

Friendly Neighborhood

Enchant land
When this Aura enters, create three 1/1 green and white Human Citizen creature tokens.
Enchanted land has "{1}, {T}: Target creature gets +1/+1 until end of turn for each creature you control. Activate only as a sorcery."

Vibrant Cityscape

{T}, Sacrifice this land: Search your library for a basic land card, put it onto the battlefield tapped, then shuffle.

Thwip!

Target creature gets +2/+2 and gains flying until end of turn. If it's a Spider, you gain 2 life.

Spider-Sense

Web-slinging {U} (You may cast this spell for {U} if you also return a tapped creature you control to its owner's hand.)
Counter target instant spell, sorcery spell, or triggered ability.

Silk, Web Weaver

Web-slinging {1}{G}{W} (You may cast this spell for {1}{G}{W} if you also return a tapped creature you control to its owner's hand.)
Whenever you cast a creature spell, create a 1/1 green and white Human Citizen creature token.
{3}{G}{W}: Creatures you control get +2/+2 and gain vigilance until end of turn.

City Pigeon

Flying
When this creature leaves the battlefield, create a Food token. (It's an artifact with "{2}, {T}, Sacrifice this token: You gain 3 life.")

Spider-UK

Web-slinging {2}{W} (You may cast this spell for {2}{W} if you also return a tapped creature you control to its owner's hand.)
At the beginning of your end step, if two or more creatures entered the battlefield under your control this turn, you draw a card and gain 2 life.

Spiders-Man, Heroic Horde

Web-slinging {4}{G}{G} (You may cast this spell for {4}{G}{G} if you also return a tapped creature you control to its owner's hand.)
When Spiders-Man enters, if they were cast using web-slinging, you gain 3 life and create two 2/1 green Spider creature tokens with reach.

Grow Extra Arms

This spell costs {1} less to cast if it targets a Spider.
Target creature gets +4/+4 until end of turn.

Web Up

When this enchantment enters, exile target nonland permanent an opponent controls until this enchantment leaves the battlefield.

Unstable Experiment

Target player draws a card, then up to one target creature you control connives. (Draw a card, then discard a card. If you discarded a nonland card, put a +1/+1 counter on that creature.)